A serological immunoassay for Hepatitis E virus ( HEV ) diagnosis 1 based on genotype 3 Open Reading frame - 2 ( ORF - 2 ) recombinant 2 proteins produced in Trichoplusia ni larvae . 3 4 5
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چکیده
1 Department of Biotechnology. INIA. Madrid, Spain. 2 Department of Infectious Pathology 9 and Epidemiology. F. Veterinaria. U. Zaragoza (Spain). 3 National Institute for Public 10 Health and the Environment. RIVM, Bilthoven (The Netherlands). 11 12 *Corresponding author: J. C. Saiz 13 Departamento de Biotecnología 14 Instituto Nacional de Investigación y Tecnología Agraria y 15 Alimentaria (INIA). 16 Ctra. Coruña Km. 7.5. 28040 Madrid (Spain) 17 E-mail: [email protected] 18 Tlph: 34-91-3471497 19 Tfax: 34-91-3572293 20 21 RUNNING TITLE: HEV ORF2 genotype 3 based ELISA 22 23
منابع مشابه
Serological immunoassay for detection of hepatitis E virus on the basis of genotype 3 open reading frame 2 recombinant proteins produced in Trichoplusia ni larvae.
Hepatitis E virus (HEV) is a major cause of acute hepatitis in humans, and strains of genotypes 1 and 2 are endemic in many regions with suboptimal sanitary conditions. In many industrialized countries, HEV strains of genotype 3 are highly endemic in swine, and an increased number of autochthonous infections with HEV genotype 3 strains have been reported lately. Serological studies of HEV infec...
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We reported previously on the complete sequence of hepatitis E virus (HEV) genotype 4, isolated from patients with sporadic cases of acute HEV infection in China. At least eight HEV genotypes have now been described worldwide, and further isolates await classification. Current immunoassays for the detection of anti-HEV antibodies are based on polypeptides from genotypes 1 and 2 only and may be ...
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Hepatitis E virus (HEV) is classified within the family Hepeviridae, genus Hepevirus. HEV genotype 3 (Gt3) infections are endemic in pigs in Western Europe and in North and South America and cause zoonotic infections in humans. Several serological assays to detect HEV antibodies in pigs have been developed, at first mainly based on HEV genotype 1 (Gt1) antigens. To develop a sensitive HEV Gt3 E...
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Isolates of hepatitis E virus (HEV) have recently been described from China that are distinct from Burmese, Mexican and US viruses and constitute a novel genotype (genotype 4). Here, the complete genomic sequence of a representative isolate of genotype 4 HEV, amplified directly from the stool of an acutely infected patient, is presented. Analysis of the entire sequence confirms our previous con...
متن کاملHepatitis E Virus
First indications of hepatitis E as a distinct disease, which differs from hepatitis A, were found in 1980 when sensitive and specific detection systems for hepatitis A virus (HAV) first became available and hepatitis epidemics were examined in India. Epidemiologic studies up to that time pointed to the fact that these hepatitis infections with clinically very similar course were caused by HAV ...
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تاریخ انتشار 2009